ABSTRACT
Objective: The aim of the study was to determine potential mood changes reported by Polish undergraduates during the second wave of the COVID-19 pandemic and how mood may be differ, according to gender, field and year of study. Differences in coping strategies depending on mood changes were also Investigated. Method: The study involved 1330 students (767 women and 563 men) aged 18-30 years studying various faculties at the Jozef Pilsudski University of Physical Education in Warsaw, Poland. A modified version of the the Coping Orientations to Problem Experienced University of Wales Institute of Science and Technology Mood Adjective Check List UMACL was used to identify mood changes, and brief version of the Coping Orientations to Problems Experienced COPE was used to identify the coping strategies employed. Results: A decrease in hedonic tone and energetic arousal and an increase in tense arousal was reported during the pandemic, with a deterioration in students' mood. Mood deterioration was lower in male students and sport and physical education students. Students who reported mood improvement were most likely to use positive reframing, acceptance, active coping, humour, and physical activity. Students with deteriorated mood used self-blaming, venting, denial, behavioural disengagement, and doing something else most frequently. Discussion: Female students, those studying physiotherapy, tourism and recreation, and other majors (nursing, occupational therapy, cosmetology), and first-year master's students are more likely to report negative psychological impact during the COVID-19 pandemic. The use of emotional and avoidance coping strategies may increase the affective cost of pandemic stress. (PsycInfo Database Record (c) 2022 APA, all rights reserved)
ABSTRACT
To (re)define tissue architecture of the lung and airways at the cellular and molecular level, we profiled five proximal-to-distal locations of healthy human lungs in depth using multi-omic single cell/nuclei and Visium Spatial Transcriptomics. Using computational data integration and analysis, we extend beyond the suspension cell paradigm of lung atlases to date, to define and discover macro and micro-anatomical tissue compartments. We describe novel cell types and states in vascular, stromal and nerve bundle microenvironments. From our spatial transcriptomics, we discover and validate a novel survival niche for IgA plasma cells in the airway submucosal glands (SMG). In this niche we define a supporting role for SMG epithelial cells in mucosal immunity through recruitment and maintenance of IgA plasma, B and CD4 T cells locally at the airway SMG. We identify an immune-supporting role for SMG duct and serous cells with distinct signalling circuits to recruit B cells and IgA plasma cells, promoting longevity and antibody secretion through expression of CCL28, APRIL and IL6. We find high expression of MHC-II in SMG duct and serous cells, which are localised closely with memory CD4 T cells, suggesting local modulation of antigen specific immune responses locally at the glands. This new tissue microenvironment, which we term the “gland-associated immune niche” (GAIN) has major implications for respiratory immunity and infection response. Our single cell and spatial data is available for download and query at lungcellatlas.org.